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- *******************************************************
- * Cytochrome P450 cysteine heme-iron ligand signature *
- *******************************************************
-
- Cytochrome P450's [1,2,3] are a group of enzymes involved in the oxidative
- metabolism of a high number of natural compounds (such as steroids, fatty
- acids, prostaglandins, leukotrienes, etc) as well as drugs, carcinogens and
- mutagens. Based on sequence similarities, P450's have been classified into
- about forty different families [4,5]. P450's are proteins of 400 to 530 amino
- acids; the only exception is Bacillus BM-3 (CYP102) which is a protein of 1048
- residues that contains a N-terminal P450 domain followed by a reductase
- domain. P450's are heme proteins. A conserved cysteine residue in the C-
- terminal part of P450's is involved in binding the heme iron in the fifth
- coordination site. From a region around this residue, we developed a ten
- residue signature specific to P450's.
-
- -Consensus pattern: F-[SGNH]-x-[GD]-x-[RHPT]-x-C-[LIVMFAP]-[GAD]
- [C is the heme iron ligand]
- -Sequences known to belong to this class detected by the pattern: ALL, except
- for P450 IIB10 from mouse, which has Lys instead of Phe in the first position
- of the pattern.
- -Other sequence(s) detected in SWISS-PROT: NONE.
-
- -Note: the term 'cytochrome' P450, while commonly used, is incorrect as P450
- are not electron-transfer proteins; the appropriate name is P450 'heme-
- thiolate proteins'.
-
- -Expert(s) to contact by email: Holsztynska E.J.
- ela@netcom.uucp or netcom!ela@apple.com
- Degtyarenko K.N.
- kirill@icgeb.trieste.it
-
- -Last update: June 1994 / Text revised.
-
- [ 1] Nebert D.W., Gonzalez F.J.
- Annu. Rev. Biochem. 56:945-993(1987).
- [ 2] Coon M.J., Ding X., Pernecky S.J., Vaz A.D.N.
- FASEB J. 6:669-673(1992).
- [ 3] Guengerich F.P.
- J. Biol. Chem. 266:10019-10022(1991).
- [ 4] Nelson D.R., Kamataki T., Waxman D.J., Guengerich F.P., Estrabrook R.W.,
- Feyereisen R., Gonzalez F.J., Coon M.J., Gunsalus I.C., Gotoh O.,
- Okuda K., Nebert D.W.
- DNA Cell Biol. 12:1-51(1993).
- [ 5] Degtyarenko K.N., Archakov A.I.
- FEBS Lett. 332:1-8(1993).
-